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reads_txt.h
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27 
28 #pragma once
29 
30 #include <nvbio/io/reads/reads.h>
32 #include <nvbio/basic/console.h>
33 
34 #include <zlib/zlib.h>
35 
36 namespace nvbio {
37 namespace io {
38 
41 
44 
47 
48 // ReadDataFile from a FASTQ file
49 // contains the code to parse FASTQ files and dump the results into a ReadDataRAM object
50 // file access is done via derived classes
52 {
53 protected:
54  ReadDataFile_TXT(const char *read_file_name,
55  const QualityEncoding quality_encoding,
56  const uint32 max_reads,
57  const uint32 max_read_len,
58  const ReadEncoding flags,
59  const uint32 buffer_size = 64536u)
60  : ReadDataFile(max_reads, max_read_len, flags),
61  m_file_name(read_file_name),
62  m_quality_encoding(quality_encoding),
63  m_buffer(buffer_size),
64  m_buffer_size(buffer_size),
65  m_buffer_pos(buffer_size),
66  m_line(0)
67  {};
68 
69  // get next read chunk from file and parse it (up to max reads)
70  // this can cause m_file_state to change
71  virtual int nextChunk(ReadDataRAM *output, uint32 max_reads, uint32 max_bps);
72 
73  // fill m_buffer with data from the file, return the new file state
74  // this should only report EOF when no more bytes could be read
75  // derived classes should override this method to return actual file data
76  virtual FileState fillBuffer(void) = 0;
77 
78 private:
79  // get next character from file
80  uint8 get();
81 
82 protected:
83  // file name we're reading from
84  const char * m_file_name;
85  // the quality encoding we're using (for FASTQ, this comes from the command line or defaults to Phred33)
87 
88  // buffers input from the fastq file
89  std::vector<char> m_buffer;
92 
93  // counter for which line we're at
95 
96  // error reporting from the parser: stores the character that generated an error
98 
99  // temp buffers for data coming in from the FASTQ file: read name, base pairs and qualities
100  std::vector<char> m_name;
101  std::vector<uint8> m_read_bp;
102  std::vector<uint8> m_read_q;
103 };
104 
105 // loader for gzipped files
106 // this also works for plain uncompressed files, as zlib does that transparently
108 {
109  ReadDataFile_TXT_gz(const char *read_file_name,
110  const QualityEncoding qualities,
111  const uint32 max_reads,
112  const uint32 max_read_len,
113  const ReadEncoding flags,
114  const uint32 buffer_size = 64536u);
115 
116  virtual FileState fillBuffer(void);
117 
118 private:
119  gzFile m_file;
120 };
121 
125 
126 inline uint8 ReadDataFile_TXT::get(void)
127 {
129  {
130  // grab more data from the underlying file
132  m_buffer_pos = 0;
133 
134  // if we failed to read more data, return \0
135  if (m_file_state != FILE_OK)
136  return 0;
137  }
138 
139  return m_buffer[m_buffer_pos++];
140 }
141 
142 } // namespace io
143 } // namespace nvbio